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| Cloning, Expression and Bioinformatics Analysis of PRPS2 Gene of Banna Mini-pig (Sus scrofa) Inbred Line |
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Abstract Phosphoribosylpyrophosphate synthetases 2 gene (PRPS2) is the key enzyme of biological synthesis, which plays important role in controlling tumor development, promoting tumor cell's apoptosis, inhibiting cell proliferation and promoting spermatogenesis. In order to study the role of PRPS2 gene in pig (Sus scrofa) male sterility and the function of the protein encoded by the gene, using the PRPS2 mRNA sequences of pig and related species from GenBank as reference gene sequences, Specific primers were designed and Banna mini-pig inbred line (BMI) PRPS2 gene was amplified. qRT-PCR was applied to analysis the mRNA expression profiles of 15 important tissues. Protein sequence was used to carry out functional bioinformatics analysis. cDNA sequence of 1 282 bp (GenBank accession number: KU705637, the corresponding amino acid sequence accession number: AOC89055) of BMI PRPS2 was obtained, which encodes a protein of 318 amino acids. Tissue expression profile indicated that PRPS2 gene expressed highly in the seminal vesicle gland, and low expression in the other 14 tissues. Bioinformatics analysis indicated that PRPS2 protein molecular weight 34.83 kD, and isoelectric point 6.15, contained 2 conserved domains, no transmembrane region; no signal peptide sequences, its N-terminal and the C-terminal were hydrophilic; it had 4 types of functional active sites; the probability of being located in the cytoplasm was 89%; PRPS2 gene highly conserved in evolution. The results of the study will lay a foundation for further study of the gene about its functions in pig male sterility.
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Received: 08 October 2016
Published: 02 March 2017
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