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| Expression of JcFATA Gene in Jatropha curcas and Its Promoter Cloning and Analysis |
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Abstract Abstract Fatty acyl acyl carrier thioesterase A (FATA) plays an important role in the process of fatty acid synthesis metabolism. The expression pattern of FATA of Jatropha curcas (JcFATA) and its promoter all has not been reported. To study the expression pattern of JcFATA gene, semi-quantitative RT-PCR and qRT-PCR were applied to detect the expression specificity of JcFATA gene. The results showed that JcFATA gene could express in multiple tissues of J. curcas, especially highly expressed in roots, flowers and leaves. In addition, in order to analyze the expression pattern of JcFATA gene promoter (PJcFATA), JcFATA gene promoter was cloned firstly and inserted into a binary expression vector pCAMBIA1300G, and then the fusion expression vector of β-glucuronidase (GUS) gene drived by JcFATA gene promoter (p1300G-PJcFATA-GUS) was constructed successfully. The fusion expression vector was introduced into Arabidopsis thaliana wild type by Agrobacterium-mediated method. After effective selection for hygromycin B resistance, the resistant plants were detected by PCR and GUS histochemical analysis. PCR detection results showed that JcFATA gene promoter had been transferred into the genome of A. thaliana (JGD No. Jcr4S00539). Results of GUS histochemical staining of tissues in different development periods of A. thaliana revealed that GUS activity displayed obviously tissue specificity, with the deeper staining in roots, flowers and leaves. All above results paved the way to further function study of JcFATA gene and JcFATA gene promoter.
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Received: 26 October 2016
Published: 13 January 2017
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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