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| Polymorphisms of KLF7 Gene in Ninghai and Guangxi Yellow Chickens (Gallus gallus) and Its Association with Carcass Traits |
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Abstract Abstract Kruppel-like factor 7 (KLF7) is a factor involved in fat metabolism and its related research on growth and carcass traits in chicken (Gallus gallus) is less. In order to analyze the association between the single nucleotide polymorphism (SNPs) of KLF7 gene and carcass traits in chicken, the SNPs of the whole genome were genotyped through 600K Affymetrix Axiom Chicken SNP chip technology in Ninghai and Guangxi yellow chickens with each of 59 individuals. Within the total 38 identified SNPs, only 4 SNPs were found in exon regions of KLF7 gene. Most of the detected SNPs revealed 3 genotypes (i.e. AA, AB and BB). The χ2 test showed that the genotypic frequencies of 5 SNPs (i.e. T16936C, A18000C, C33569T, T34758C and T40474G) within Ninghai yellow chicken and 2 SNPs (i.e. A18715C and T45125A) within Guangxi yellow chicken were not in agreement with the Hardy-Weinberg equilibrium (P＜0.05), while the genotype frequencies of the other SNPs in these two chicken populations were all under the Hardy-Weinberg equilibrium (P＞0.05), which showed that there was dynamic equilibrium in two studied chicken populations. According to the classification of polymorphism information content (PIC), the polymorphism at most of the detected SNPs in the current study presented moderately in these 2 chicken breeds. Association studies between each SNP genotype and carcass traits were performed by using the GLM. The results showed that 7 SNPs (i.e. A14371G, A18000C, C28254A, C33569T, T45125A, A56295G and G59074A) of KLF7 gene in Ninghai yellow chicken and 6 SNPs (i.e. C12493G, A18000C, G30642A, A38815G, T45125A and C58906T) in Guangxi yellow chicken were significantly associated with carcass traits (P＜0.05 or P＜0.01). The 2 chicken populations possessed 2 common SNPs (i.e. A18000C and T45125A) which significantly associated with carcass traits (P＜0.05 or P＜0.01). The 2 SNPs (i.e. A18000C and T45125A) revealed 3 genotypes (i.e. AA, AB and BB) in Ninghai yellow chicken, while 2 genotypes (i.e. AB and BB) in Guangxi yellow chicken . Genotype AB of the site A18000C was significantly superior to BB in breast muscle rate in Ninghai yellow chickens (P＜0.01), while the AB genotype of T45125A was significantly superior to AA and BB in semi-eviscerated rate, eviscerated rate, abdominal fat weight and abdominal fat rate (P＜0.05 or P＜0.01). Genotype AB of the site A18000C was significantly superior to BB in breast muscle rate in Guangxi yellow chickens (P＜0.05), while the AB genotype of T45125A was significantly superior to BB in slaughter rate, semi-eviscerated rate, eviscerated weight and eviscerated rate (P＜0.05). The results suggested that KLF7 gene may be one of the candidate genes that control carcass traits, and 2 SNPs (i.e. A18000C and T45125A) could be potential candidate genetic markers for Marker-aid selection in chickens.
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Received: 04 October 2016
Published: 13 January 2017
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