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| Cloning and Sequence Analysis for Coat Protein Gene of ZYMV Infecting of Melon (Cucumis melo) in Xinjiang |
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Abstract Xinjiang is famous for fruit in China. And the melon is not only one of the most iconic brand, but the economic pillar industries that increase the farmers' income of home at recent years. However, due to the Xinjiang melon have been threatened increasingly by virus disease, causing a large area yield reduction even total loss of the melon yield when serious. It is affected seriously the farmers' production enthusiasm and the improvement of standard of living. And Zucchini yellow mosaic virus (ZYMV) is one of the most representative virual. In order to research biological control and anti-viral melon (Cucumis melo) in different cultivars region of Xinjiang, homologous cloning method were used, the coat protein (CP) complete gene sequence of ZYMV from 19 different regions of Xinjiang were amplified. The CP complete gene sequences of ZYMV were 1 080~1 180 bp, encoding 253~301 amino acids and synthetic protein size was approximately 33 kD. The result indicated that the nucleic acid sequence of 3' and 5' terminal noncoding region were less conserved, but the coding region and the surrounding sequence were high conserved. The N terminal of the amino acids was more conserved than C terminal. According to ZYMV homology results, isolator from 19 regions belonged to the same genotype of ZYMV. Through the analysis of the evolutionary tree that constructed according to nucleic acid sequences, ZYMV were divided into 4 subgroups, and the basis of subgroups were divided compliance with viral distribution character. The physical and chemical properties of proteins, such as the kinetics of protein, chemical and thermodynamic stability results showed that CP of ZYMV belonged to stable protein. The combined analysis with average summer precipitation and mean temperature trending in Xinjiang results showed that the change trends between index of hydrophobic and fat in CP of ZYMV with the corresponding region had a certain correlation. The analysis results indicated that the stability CP of ZYMV had some differences in different region, which may be related to the particular climatic conditions and geographic environment in Xinjiang. By sequencing analysis this study obtained the nucleic acid sequence and characteristics of CP in ZYMV from 19 regions in Xinjiang. This study will offer a theoretical foundation for the biopesticide research, and cultivating anti-virus melon in future by using RNA interference technology or genomic editing techniques. Meanwhile, It can provide initial direction for conducting the pathogenic mechanism of plant viruses and the spread of the virus to explore the research environment and propagation mechanism.
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Received: 09 October 2015
Published: 01 April 2016
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