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| Functional Analysis of STMP in the Process of the Branch Development in Arabidopsis thaliana |
| 1, 1, 1,wang fengru1, |
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Abstract Arabidopsis thaliana steroidogenic acute regulatory related lipid transfer (START) domain containing membrane related protein (STMP) is a function unknown protein containing transmembrane segments, it has 440 amino acid. From 84nd to 295th amino acids is START conserved domain. There is a phosphatidycholine (PtdCho) binding site in START domain. In order to clarify the role of STMP in the development and regulation of Arabidopsis thaliana, this study used the 35S promoter to construct the START domain, Agrobacterium tumefaciens was used to infect the expression vector of STMP in wild type Arabidopsis thaliana, obtained the transgenic Arabidopsis thaliana and used qRT-PCR technology to verify it; Clear the role of STMP in the process of the regulation of the branch of Arabidopsis thaliana through analysis of the developmental status of the transgenic Arabidopsis thaliana; Analysis of temporal and spatial expression of STMP by qRT-PCR technique; Preliminary analysis of the subcellular localization of STMP was performed through the establishment of STMP-GFP (green fluorescent protein) fusion expression vector and protoplast transformation. In this study, transgenic lines over-expressing STMP were obtained. The expression of STMP in transgenic plants was significantly higher than that in wild type, and the temporal and spatial expression of STMP showed that the expression of STMP in stem was the highest, Green GFP localized on cell membrane and cell cytoplasm. These results showed that STMP promotes the development of the branches in Arabidopsis thaliana, This study has important significance to improve the regulatory mechanism of Arabidopsis thaliana branching, and provide a theoretical basis for the design of directional plant type.
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Received: 16 September 2015
Published: 24 February 2016
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Corresponding Authors:
wang fengru
E-mail: fengruwang@yahoo.com.cn;wfr15931945160@126.com
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