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| Screening, Identification of a High-producing Cold-active Amylase Strain and Its Amylase Characterization |
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Abstract Strains producing cold-active amylase were isolated from the melt water of Yangbark glacier, East Pamirs, amongthem, the strain D5-2 with the highest amylase activity was identified as Pseudomonadaceae sp.by16S rDNA sequence analysis. The study on enzymatic properties showed that the optimum temperature and pH for catalytic activity was 25℃ and pH 6.5, respectively, but the thermal stability of the enzyme is relatively poor. The cold-adaptable amylase had high activities between pH 5.5 and 8.0. The amylase activity was activated by Mg2+ang Mn2+, and was inhibited by Fe2+, Na+and EDTA.The result indicates Pseudomonadaceae sp.D5-2 is a high-producing cold-active amylase strain which shows research and development potential, it deserves to be further researched.
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Received: 10 September 2015
Published: 05 February 2016
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