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| The Development of Molecular Markers Closely Linked to TuMV Resistance Gene TuRBCS01 in Chinese Cabbage (Brassica campestris ssp. pekinensis) |
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Abstract Virus disease is one of the 3 major diseases in the production of Chinese cabbage (Brassica campestris ssp. pekinensis), it has a strong impaction to the production and quality of Chinese cabbage. The virus disease in Chinese cabbage was resulted by 3 viruses alone or compound, which were Turnip mosaic virus (TuMV), Cucumber mosaic virus (CMV) and Tobacco mosaic virus (TMV). TuMV was the main pathogen. Therefore, to breed TuMV resistant varieties became the main target. Molecular marker assisted selection can greatly speed up the process of breeding. Because of the complexity of the genetic law for resistance to virus of Chinese Cabbage. At present, the location of the resistant gene and the screening of the linkage markers can not meet the needs of breeding. In order to make better molecular marker assisted selection for virus resistance of Chinese cabbage (Brassica campestris ssp. pekinensis), we screened closely linked molecular markers for resistance to TuMV in Chinese Cabbage. Based on the mapping of that gene before, BC1 segregation population, BSA method, SSR and SSP technology techniques were used to develop molecular markers. Among 13 SSP and 16 SSR primers, 8 showed polymorphic between the 2 parents, markers amplified using 5 primers linked with gene TuRBCS01, which were mBr4072, Bra025493-1, Bra025493-2, Bra025467-4 and Bra025467-5. Two markers were developed closely linked to gene TuRBCS01, which were SAAS_mBr4072_240 (1.5 cM) and Bra025493-1(1.0 cM), respectively. In addition, SSP markers SAAS_ Bra025493-2_749, SAAS_Bra025467-4_780 and SAAS_Bra025467-5_956 were found co-segregation with that gene TuRBCS01. The markers have enriched the number and types of molecular markers about Chinese cabbage's resistance to TuMV, and they are expected to be used as marker-assisted selection in Chinese cabbage's resistance to virus disease.
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Received: 19 June 2015
Published: 29 December 2015
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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