转基因大豆MON89788基体标准物质的研制

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Abstract Genetically modified organism (GMO) ingredients detection is an important part of GMO safety management and GMO labeling, GMO detection reference material (RM) is guaranteed to get accurate, reliable, comparable GMO ingredients detection results. GM soybean (Glycine max) MON89788 is a GM plant that has been approved to import and use as in-process raw material in China. Therefore, preparation of GM soybean MON89788 RM is essential for its safety management. In this study, GM soybean MON89788 and traditional soybean seeds A3244 were milled using a cryo-grinding vibrating mill separately, then the GM soybean MON89788 matrix RM was prepared by weighting and mixing of GM and Non-GM powder. The value of MON89788 matrix RM was 49.3 g/kg in mass fraction. The mixture was further dispensed into brown bottles. Analysis of variance (F test) was performed to test homogeneity of RMs. The calculated F-statistics is 1.618, less than the critical value F (0.05, 11, 24). The results indicated that there was no significant difference between bottles and within bottles, and the RMs were homogeneous. Stability study results indicated the RMs could be stored under ambient conditions for 4 weeks, and stored at - 20 ℃ for 30 months or more. The certified value was assigned based on mass fraction of GMO powder and non-GMO powder with the units of g/kg, and mass fraction of GMO DNA and non-GMO DNA with the units of ng/μg. The certified value was expressed as (49.3±2.0) g/kg, and (49.8±5.9) ng/μg. This batch of RMs was 1.0 g/bottle, and the recommended minimum sample intake was 100 mg. The RMs could be used in qualitative and quantitative detection of GM soybean MON89788, as well as in standardization of GMO detection method and laboratory quality control. This work provides theoretical basis and technical support for the development of GM detection matrix RMs.

Key words： Genetically modified soybean MON89788 Matrix reference material Value characterization qRT-PCR

Received: 27 July 2015 Published: 29 December 2015

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	Articles by authors
	ZHANG Li
	SONG Gui-Wen
	CHEN Beng
	LI Wen-Long
	WU Yu-Hua
	WU Gang

Cite this article:

ZHANG Li,SONG Gui-Wen,CHEN Beng, et al. Development of Genetically Modified Soybean (Glycine max) MON89788 Matrix Reference Material [J]. , 2016, 24(2): 295-304.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2016/V24/I2/295

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