Abstract The aim of this study was to analyze the expression profile of the CD8B gene in different tissues of pigs (Sus scrofa), and to reveal the genetic effect of the CD8B gene polymorphism on blood immune traits. The expression level of the CD8B gene in seven tissues of Large White pigs was quantified by fluorescence quantitative polymerase chain reaction, and gene polymorphism was detected by direct sequencing and polymerase chain reaction-restriction fragment length polymorphism method in 382 Large White, 84 Landrace and 90 Songliao Black pigs, and effects of the CD8B gene mutation on peripheral blood T lymphocyte subsets and hematological traits were analyzed in the Large White samples. The results showed that the highest CD8B mRNA was expressed in the spleen, and a missense mutation (c.602 G>A) was found in exon 5 of the CD8B gene, and three genotypes (AA, AG and GG genotypes) were all detected in Large White, Landrace and Songliao Black populations. The CD8B gene polymorphism was significantly associated with blood CD4＋CD8－, CD4
XU Jin-Gen,YU Tong-Long,CHE Zhuan-Yan, et al. Expression Profile, Polymorphism Detection and Association of the CD8B Gene with Blood Immune[J]. , 2016, 24(3): 357-365.
胡永明, 赵明秋, 陈立军, 等. 2011. 猪瘟病毒感染对猪外周血T淋巴细胞亚群及TNF-α和IFN-γ的影响[J]. 中国预防兽医学报, 33(2): 126-129. (Hu Y M, Zhao M Q, Chen L J, et al. 2011. Effect of classical swine fever virus infection on the T lymphocyte subpopulations in peripheral blood and changes of alpha-interferon and gamma-interferon of pigs[J]. Chinese Journal of Preventive Veterinary Medicine, 33(2): 126-129.) 孙广力, 蔡雪辉, 郑世民, 等. 2011. 高致病性猪繁殖与呼吸综合征病毒感染仔猪外周血细胞的动态变化[J].中国兽医杂志, 47(7): 13-15. (Sun G L, Cai X H, Zheng S M, et al. 2011. Dynamic changes of blood cells from piglets infected with high pathogenic porcine reproductive and respiratory syndrome virus[J]. Chinese Journal of Veterinary Medicine, 47(7): 13-15.) 王超, 赵书红, 朱猛进. 2014. 猪抗病育种的相关问题及研究进展[J]. 中国畜牧杂志, 50(22): 67-72. (Wang C, Zhao S H, Zhu M J. 2014. Related problems and research progress in anti-disease breeding of pig[J]. Chinese Journal of Animal Science, 50(22): 67-72.) 王怀中, 林松, 王彦平, 等. 2014. 品种和母猪对仔猪血常规和T淋巴细胞亚群性状的效应分析[J]. 畜牧兽医学报, 45(11): 1760-1766. (Wang H Z, Lin S, Wang Y P, et al. 2014. Analyses of breeds and litter effect on routine blood traits and T-lymphocyte subpopulation traits in piglets[J]. Acta Veterinaria et Zootechnica Sinica, 45(11): 1760-1766.) 王生富, 景志忠, 陈国华, 等. 2008. 猪CD8β基因的克隆、表达及其结构与功能分析[J]. 中国预防兽医学报, 30(9): 737-742. (Wang S F, Jing Z Z, Chen G H, et al. 2008. Cloning, expression and structure and function analysis of porcine CD8β gene[J]. Chinese Journal of Preventive Veterinary Medicine, 30(9): 737-742.) 周向梅, 高得仪, 王清兰, 等. 1999. 仔猪断奶应激对血液和生化的影响[J]. 中国兽医杂志, 25(9): 6-8. (Zhou X M, Gao D Y, Wang Q L, et al. 1999. Influence of weaning stress on blood and biochemical profiles in pigs[J]. Chinese Journal of Veterinary Medicine, 25(9): 6-8.) 左之才, 崔恒敏, 彭西, 等. 2013. 胸膜肺炎放线杆菌感染对猪外周血T淋巴细胞亚群及增殖影响[J]. 东北农业大学学报, 44(3): 30-34. (Zuo Z C, Cui H M, Peng X, et al. 2013. Effect of Actinobacillus pleuropneumoniae infection on proliferation and subsets of T-Lymphocyte in peripheral blood of the swine[J]. Journal of Northeast Agricultural University, 44(3): 30-34.) Bosselut R, Kubo S, Guinter T, et al. 2000. Role of CD8β domains in CD8 coreceptor function: importance for MHC I binding, signaling, and positive selection of CD8+ T cells in the thymus[J]. Immunity, 12(4): 409-418. Devine L, Thakral D, Nag S, et al. 2006. Mapping the binding site on CD8β for MHC class I reveals mutants with enhanced binding[J]. The Journal of Immunology, 177(6): 3930-3938. Erkens T, Van Poucke M, Vandesompele J, et al. 2006. Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGC1A[J]. BMC Biotechnology, 6: 41. Flori L, Gao Y, Lalo? D, et al. 2011. Immunity traits in pigs: substantial genetic variation and limited covariation[J]. PLoS ONE, 6(7): e22717. Irie H Y, Mong M S, Itano A, et al. 1998. The cytoplasmic domain of CD8β regulates Lck kinase activation and CD8 T cell development[J]. The Journal of Immunology, 161(1): 183-191. Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2 -△△CT method[J]. Methods, 25: 402-408. Lu X, Fu W X, Luo Y R, et al. 2012. Genome-wide association study for T lymphocyte subpopulations in swine[J]. BMC Genomics, 13:488. Renard V, Romero P, Vivier E, et al. 1996. CD8β increases CD8 coreceptor function and participation in TCR-ligand binding[J]. Journal of Experimental Medicine, 184(6): 2439-2444. Visscher C H, Janss L L, Niewold T A, et al. 2002. Disease incidence and immunological traits for the selection of healthy pigs. A review[J]. Veterinary Quarterly, 24(1): 29-34. Wang J Y, Luo Y R, Fu W X, et al. 2013. Genome-wide association studies for hematological traits in swine[J]. Animal Genetics, 44(1): 34-43. Wang W W, Liu Y, Fu W X, et al. 2015. Single-nucleotide polymorphisms in CD8A and their associations with T lymphocyte subpopulations in pig[J]. Molecular Genetics and Genomics, 290(4): 1447-1456. Zhang Z Y, Hong Y, Gao J, et al. 2013. Genome-wide association study reveals constant and specific loci for hematological traits at three time stages in a White Duroc × Erhualian F2 resource population[J]. PLoS ONE, 8(5): e63665.
