猪CD8B表达谱、基因多态性及其与血液免疫性状的关联分析

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Abstract The aim of this study was to analyze the expression profile of the CD8B gene in different tissues of pigs (Sus scrofa), and to reveal the genetic effect of the CD8B gene polymorphism on blood immune traits. The expression level of the CD8B gene in seven tissues of Large White pigs was quantified by fluorescence quantitative polymerase chain reaction, and gene polymorphism was detected by direct sequencing and polymerase chain reaction-restriction fragment length polymorphism method in 382 Large White, 84 Landrace and 90 Songliao Black pigs, and effects of the CD8B gene mutation on peripheral blood T lymphocyte subsets and hematological traits were analyzed in the Large White samples. The results showed that the highest CD8B mRNA was expressed in the spleen, and a missense mutation (c.602 G>A) was found in exon 5 of the CD8B gene, and three genotypes (AA, AG and GG genotypes) were all detected in Large White, Landrace and Songliao Black populations. The CD8B gene polymorphism was significantly associated with blood CD4＋CD8－, CD4

Key words： Pig Cluster of differentiation 8B gene (CD8B) Gene expression SNP Blood immune traits

Received: 31 July 2015 Published: 05 February 2016

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	Articles by authors
	XU Jin-Gen
	YU Tong-Long
	CHE Zhuan-Yan
	SA Yi-Hua
	LI Sheng-He
	ZHANG Qi

Cite this article:

XU Jin-Gen,YU Tong-Long,CHE Zhuan-Yan, et al. Expression Profile, Polymorphism Detection and Association of the CD8B Gene with Blood Immune[J]. , 2016, 24(3): 357-365.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2016/V24/I3/357

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