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| Effects of Baicalin on the Development of Mouse (Mus musculus) Embryos Cultured In vitro |
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Abstract Baicalin is a kind of flavonoid, can promotes posttransplant fetal growth and development, and hasn't toxicity to the fetus development. The objectives of the present study were to investigate the effects of baicalin on the development of mouse (Mus musculus) embryos in vitro and it's possible molecular mechanism. Mouse embryos cultured in vitro were randomly divided into control group(CZB cultural medium) and experimental group (supplemented with 4 μg/mL of baicalin). The development rates of embryos in their different stages were recorded, respectively, using a stereoscopic microscope, and also the nuclei of blastocysts were stained with orcein for cell number counting. The expressions of gap junction protein alpha-1(GJA1), heat Shock 70 (Hsp70), B-cellymphoma/leukemia-2(Bcl-2) and Bcl-2 associated X protein (Bax) mRNA were detected by using qRT-PCR. The results showed that baicalin treatment, as compared to control group, significantly improved (P＜0.05) 2-cell and 4-cell embryonic cleavage rates, morula, blastocyst and expanded blastocyst development rates and blastocyst hatching rates, and also significantly (P＜0.05) improved the cell number of expanded and hatched blastocysts. In addition, the expression of GJA1 mRNA was significantly increased (P＜0.05), and the expressions of Hsp70 and Bax mRNA were significantly decreased (P＜0.05), and also the ratio between Bax/Bcl-2 was significantly reduced (P＜0.05). We concluded that baicalin improves the blastocyst development rates and the quality of blastocysts of mouse embryo in vitro, probably, by upregulating GJA1 mRNA expression and downregulating Hsp70 and Bax mRNA expressions. The study reveals the possible mechanism of action that the baicalin promotes embryonic development, and provides basic data for the study of baicalin tocolysis role.
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Received: 07 April 2015
Published: 11 October 2015
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