甜瓜细菌性叶枯病菌胶体金ICA检测试纸条的研发与应用

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Abstract Bacterial leaf spot blight and bacterial fruit blotch are the two main bacterial diseases in muskmelons (Cucumis melo). They always infect the muskmelons simultaneously with the similar symptoms, and it is difficult to use traditional methods to distinguish the two bacterial diseases. In this study, a rapid and simple immun ochromatography assay (ICA)for in-site detection of Pseudomonas syringae pv. lachrymas in muskmelons was developed using a new anti-Pseudomonas syringae pv. lachrymas monoclone antibody. In qualitative detection, the detection limit was determined as 2.5×103 cfu/mL. Specificity results showed that there was no cross-reactivity to Acidovorax citrulli which indicated the good specificity of the ICA. In the quantitative detection, the detection results of Pseudomonas syringae pv. lachrymas were scanned by a membrane strip reader, and a standard inhibition curve and calibration curve were established, the reading value ratio of T and C line had a remarkable linear relation with logarithm of Pseudomonas syringae pv. lachrymas concentration, the regression equation was y=0.3922x-1.263 8, R2=0.988 1. Therefore, the established high selective ICA was a very practical method for rapid, simple and low-cost detection of Pseudomonas syringae pv. lachrymas, and not only achieved in situ rapid detection of Pseudomonas syringae pv. lachrymas in the field, but also distinguished Acidovorax citrulli effectively, which was helpful for the development of melon planting industry.

Key words： Muskmelon bacterial leaf blight Monoclonal antibody Immunochromatographic assay (ICA)

Received: 30 March 2015 Published: 23 November 2015

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	Articles by authors
	MU Lin-Yu
	YU Li-Min
	TIAN Yan-Li
	DIAO Yu-Jiang
	HU Bai-Shi

Cite this article:

MU Lin-Yu,YU Li-Min,TIAN Yan-Li, et al. Development and Application of a Colloidal Gold ICA Test Strip for Detection of Muskmelon (Cucumis melo) Bacterial Leaf Spot Blight[J]. , 2016, 24(1): 134-141.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2016/V24/I1/134

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