Abstract In order to analyze genetic polymorphisms of Allium fistulosum CMS (cytoplasmic male sterile) lines and maintainer, 3 mitochondrial gene probes ATP synthase F0 subunit 6 (atp6), NADH dehydrogenase subunit 5 (nad5) and cytochrome c oxidase subunit 3 (cox3), combined with 2 restriction enzymes HindⅢ and BamHⅠ were used to detect the polymorphism of mitochondrial genomes. The Southern blot results indicated that mitochondrial genome had polymorphism between the CMS lines and the maintainer lines. By using the combination of HindⅢ/nad5 in the RFLP (restriction fragment length polymorphism) experiment, 5 CMS lines showed 1 specific band of 10 kb，and the 4 maintainer lines showed another specific band of 7 kb. In summary, RFLP (HindⅢ/nad5) could be used to identify the type of cytoplasm in Allium fistulosum. These results may help to reveal the molecular mechanisms underlying the CMS and will be useful for mitochondrial gene cloning studies of the Allium fistulosum.
YU Chan,DIAO Hong,ZHANG Li-Yang, et al. RFLP Analysis of Mitochondrial Genomes of Cytoplasmic Male Sterile Lines and Maintainer Lines in Welsh Onion (Allium fistulosum L.)[J]. , 2015, 23(7): 888-893.
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