绵羊抵抗素基因(RETN)克隆、序列分析及其在饥饿模型中的表达变化规律

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Abstract Resistin (RETN) is a specifically produced hormone by fat cells, which plays an important role in glucose and lipid metabolism and also is one of popular candidate genes in animal fat deposition and metabolism. Aiming at study the expression pattern of RETN gene in rump-fat deposition and metabolism of sheep (Ovis aires), long fragment PCR, bioinformatics method and semi-quantitative RT-PCR were used to amplify the full-length sequences, analyze the sequences and detect gene expression profiling of sheep RETN gene, respectively. Fluorescence quantitative Real-time PCR (qRT-PCR) method was used to analyze the expression change of RETN gene between starving and its control groups based on starving model in Altay sheep. The results of cloning and sequence analysis showed that RETN gene encoded 109 amino acids (GenBank No. KJ704841), which was different from the published RETN sequence in GenBank database. RETN mRNA expression profiling showed that RETN expressed in liver, spleen, lung and rump fat differently, especially highly in the liver, significantly higher than that of other groups (P＜0.01), which indicated that RETN gene might play important physiological and biochemical functions in fat synthesis and metabolism in the liver. The result of qRT-PCR showed that RETN mRNA significantly differentially expressed in between starving group and its control groups, with a expression level in persistent starvation group of 5.2 times than that of control group, suggesting that RETN gene might play an important role in fat mobilization. The above findings provide fundamental data for further study on RETN biological function in fat-rump sheep.

Key words： Resistin (RETN) Starving model Rump-fat Sequence analysis Expression profiling research

Received: 04 February 2015 Published: 01 June 2015

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2015/V23/I8/981

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