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| Study on Polymorphism and Expression of Follicile Stimulating Hormone Receptor Gene (FSHR) in Guizhou Native Goat (Capra hircus) |
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Abstract This experiment was conducted to reveal the effect of the polymorphism of exon 1 and 10 of follicile stimulating hormone receptor gene (FSHR) on the reproductive traits of Qianbei Ma goat (Ovis Linnaeus). The expression of FSHR gene in hypothalamus, pituitary, uterus, fallopian tubes and ovaries was studied using high prolificacy goat breed (Qianbei Ma goat and Guizhou White goat) and low prolificacy goat breed (Guizhou Black goat) as a control. The single nucleotide polymorphism (SNP) of FSHR gene in exon 1 and 10 was detected by direct sequencing technology of PCR products and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), and different expression in Guizhou native goat 5 tissues was detected by quantitative Real-time PCR (qRT-PCR). The result showed that 3 SNPs locus (1-C126T,10-C1246A and 10-A1412C) were deteced in exon1 and 10 in Qianbei Ma goat, and 10-A1412C was a sense mutation and devided into 3 genotypes: CC, AC and AA, and the genotypes frequency were 0.472 7, 0.309 0 and 0.218 3, respectively, the populations was in high polymorphism situation. The relationships between reproductive trait of litter size of Qianbei Ma goats and genotype CC and AC were significantly higher than those with genotype AA (P＜0.05). qRT-PCR result showed that the expression level of FSHR was high in hypothalamic but low in ovarian ducts, and with no significant difference in hypothalamus, pituitary, uterus, fallopian tubes and ovaries in 3 breeds goats (P＞0.05). This study indicates that FSHR gene may be a potential major gene or link to major gene effecting goat litter sizes, and a candidate molecular marker for reproductive traits selection in Qianbei Ma goats.
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Received: 04 September 2014
Published: 13 April 2015
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