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| Tissue Expression Profile and Expression Pattern of Myogenin Gene(MyoG) in Jinghai Yellow Chicken (Gallus gallus) |
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Abstract Myogenin gene (MyoG) is a member of myogenic regulatory factors (MRFs) family and plays an important role in regelation of muscle building. To explore the expression profile and expression pattern of MyoG gene and its possible mechanism of action, a pair of fluorescence quantitative primers according to the cloned coding sequence (CDS) of MyoG in Jinghai yellow chicken (Gallus gallus) was designed. Real-time fluorescent quantitative PCR was used to detect the expression level of MyoG in different tissues to draw the expression profile. Then, the expression levels of MyoG gene in high expression tissue of breast and leg muscle at four time points were detected to study the expression pattern. The results showed that MyoG mainly expressed in breast and leg muscle and the expression level was extremely significant higher (P＜0.01) than that in other tissues. Expression of MyoG was also detected in heart and ovary, however, the expression level was very low. Expression pattern of MyoG showed a change trend. The variation of MyoG expression in breast and leg muscle of female chicken was stable. The expression in breast and leg muscle of male chicken showed significant changes. Expression of MyoG gene achieved the highest level at 4 weeks of age and lowest level at 0 week of age. For male and female chicken, expression level of MyoG gene in breast muscle was higher than that in leg muscle. This study revealed the expression profile and expression pattern with the growth and development of muscle of Jinghai yellow chicken, and might lay a foundation for further study on mechanism of action and expression regulation of MyoG gene.
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Received: 22 September 2014
Published: 17 March 2015
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