|
|
|
| Influence of Sludge Retention Time (SRT) on Phosphorus Removal and Microbial Community Structure of Acidobacteria |
| 2, 2, 2, 2, 2 |
|
|
|
|
Abstract Acidobacteria is an important phosphorus removal bacteria in the sequencing batch reactors (SBR) system, and its effects on phosphorus removal process should not be ignored. Sludge retention time (SRT) is an essential parameter of the design and operation of the SBR process. If the SRT changed, the phosphorus removal effect of SBR would also change. In order to inspect the phosphorus removal effect, the SRT of SBR system was tested for 10, 15, 20 and 30 d. PCR-denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the microbial community structure of acidobacteria in the activated sludge. The results indicated that the removal rate of total phosphorus reached 90%, 74%, 52% and 31% at the sludge of 10, 15, 20 and 30 d, respectively. The removal effect was the best at the sludge of 10 d, and short sludge age was more advantageous to the total phosphorus removal. Acidobacteria was sensitive to the changes of the sludge age conditions, and the dominant strains of the Acidobacteria changed frequently. The diversity index reached 0.87 at the sludge of 10 d, and dropped to 0.81 at the sludge of 30 d. The results indicate that the Acidobacteria is more adapting to the environment of long sludge age.
|
|
Received: 29 October 2014
Published: 17 March 2015
|
|
|
|
| [1] 赵耘挚，刘振鸿. SBR工艺脱氮除磷研究进展[J]，工业用水与废水，2002，33（4）7-9.[2] 牟洁, 孙宝盛, 陈谊. 利用PCR-DGGE研究膜生物反应器中微生物的群落结构[J]，环境科学学报, 2010, 30(4): 729-734.[3] 范艳明，孙宝盛，张 斌，等. 小试膜生物反应器(MBR)中活性污泥微生物种群结构的演变[J]，环境化学，2013，32(6):1046-1048.[4] Namba A．, Shigenobu Y．, Kobayashi M．, et al. A new primer for 16S rDNA analysis of microbial communities associated with Porphyra yezoensis[J]. Fisheries Science, 2010, 6(5): 873-878.[5] Sanz J．L．, Kochling T．，Molecular biology techniques used in wastewater treatment: An overview[J]. Process Biochemistry, 2007, 42(2):119-133.[6] Rosenberger S．, Laabs C．, Lesjean B．, et al. Impact of colloidal and soluble organic material on membrane performance in membrane bioreactors for municipal wastewater treatment. [J]，Water Research, 2006, 40(4): 710-720.[7] 于凤庆, 孙宝盛, 陈 谊, 等. MBR活性污泥培养驯化过程中生物多样性研究[J]. 环境科学学报, 2012, 32(9): 2084-2090.[8] Nicolaiscn M．H．, Ramsing N．B．. Denaturing gradient gel electrophoresis (DGGE) approaches to study the diversity of ammonia-oxidizing bacteria[J]. Journal of Microbiological Methods, 2002, 50(2): 189-203.[9] 李亚静，孙力平，李计元. SBR法处理低碳源城市污水除磷脱氮效果及规律研究[J]，天津城市建设学院学报，2006，12(2)：112-115．[10] 李 楠．SBR系统在低温条件下的废水生物除磷性能及除磷途径分析[D]，哈尔滨：哈尔滨工业大学市政环境工程学院，2010. |
|
|
|
