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| Alternative Splicing and Expression Pattern Analyses of Two MADS-BOX Genes AGL6 and FUL1 in Brachypodium distachyon |
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2. Northwest A&F University
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Abstract MADS-BOX gene family is one kind of important transcription factors which regulates multiple developmental processes in plant, and AGAMOUS LIKE 6(AGL6) and FRUITFULL(FUL) genes belong to its subfamily. In this study, cDNA sequences of two Brachypodium distachyon MADS-BOX genes BdAGL6 and BdFUL1 were cloned. Furthermore, the alternative splicing and expression pattern of both genes were analyzed. As a result, three BdAGL6 transcripts were cloned. Further analysis showed three isoforms were generated by alternative splicing through intron retention mechanism. BdAGL6-3 was conservative with AGL6 transcription factors in rice (Oryza sativa) and maize (Zea maize). Compared with BdAGL6-3, additional 7-aa and 5-aa were inserted in the C domain of BdAGL6-1 and BdAGL6-2, respectively. BdAGL6 strongly expressed in flowers, and the expression strength of BdAGL6-3 was the strongest while the expression strength of BdAGL6-1 and BdAGL6-2 were weaker. Meanwhile, alternative splicing also occurred in BdFUL1 too, and two transcripts of BdFUL1-1 and BdFUL1-2 with similar expression pattern were generated, and both of them expressed in flowers, stems and root of seedlings. These findings provide data for further functional elucidation of these Brachypodium distachyon MADS-BOX gene family.
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Received: 24 September 2014
Published: 01 March 2015
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