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Abstract Cytochrome P450 enzymes (CYPs) are involved in many physiological functions in insects, such as the metabolism of signal molecules, adaption to host plants and insecticide resistance. Species of Lepidoptera, which include the most disruptive agricultural pests, rank only secondly to those of Coleoptera. On basis of the draft genome sequence of lepidopteran insect, Manduca sexta L., a genome-wide analysis of CYPs was performed and 110 CYP-related sequences were obtained, which could be classified into 29 families according to standard nomenclature, a lot of which were tandemly arranged on chromosomes. We furtherly compared the CYP-related sequences with those of monarch butterfly (Danaus plexippus L.) and the model insect silkworm (Bombyx mori L.). The result revealed that there were 12 pairs of orthologs in CYP2 and mitochondrial clans, whereas many CYPs in CYP3 and CYP4 clans were present with species-speci?c expansions. There were more orthologous CYP pairs among lepidopteran insects in comparsion with those between lepidopteran insects and the other insect orders. Many orthologous groups of paralogous genes could be also distinguished from the phylogenetic tree in spite that a high frequency of species-specific expansion, relatively few different CYP families and subfamiles were represented among different lepidopteran insects. Extensive synteny of CYPs was also maintained in the different lepidopteran insect genomes. The insights obtained from comparative genomic analysis will greatly facilitate researches on all lepidopteran CYPs, and in particularly on selective targets for innovative pest management.
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Received: 25 August 2014
Published: 13 January 2015
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| [1]艾均文, 王根洪, 李艳红, 余泉友, 张学松, 朱勇, 向仲怀.家蚕基因的克隆、序列分析及转录活性[J].昆虫学报, 2008, 51(3):237-245[2]艾均文, 薛宏, 何行健, 孟繁利, 朱勇, 向仲怀.家蚕细胞色素基因的研究进展[J].昆虫学报, 2011, 54(8):918-926[3]Ai JW, Yu QY, Cheng TC, Dai FY, Zhang XS, Zhu Y, Xiang ZH.Characterization of multiple CYP9A genes in the silkworm,Bombyx mori[J].Mol Biol Rep, 2010, 37(3):1657-1664[4]Ai JW, Zhu Y, Duan J, Yu QY, Zhang GJ, Wan F, Xiang ZH.Genome-wide analysis of cytochrome P450 monooxygenase genes in the silkworm,Bombyx mori[J].Gene, 2011, 480(1-2):42-50[5]Daimon T, Kozaki T, Niwa R, Kobayashi I, Furuta K, Namiki T, Uchino K, Banno Y, Katsuma S, Tamura T, Mita K, Sezutsu H, Nakayama M, Itoyama K, Shimada T, Shinoda T.Precocious Metamorphosis in the Juvenile Hormone–Deficient Mutant of the Silkworm,Bombyx mori[J].PLoS Genet, 2012, 8(3):e1002486-[6]d'Alencon E, Sezutsu H, Legeai F, Permal E, Bernard-Samain S, Gimenez S, Gagneur C, Cousserans F, Shimomura M, Brun-Barale A, Flutre T, Couloux A, East P, Gordon K, Mita K, Quesneville H, Fournier P, Feyereisen R.Extensive synteny conservation of holocentric chromosomes in Lepidoptera despite high rates of local genome rearrangements[J].Proc Natl Acad Sci U S A., 2010, 107(17):7680-7685[7]Feyereisen R.Arthropod CYPomes illustrate the tempo and mode in P450 evolution[J].Biochimica et Biophysica Acta, 2011, 1814(1):19-28[8]Feyereisen R.Insect cytochrome P450. in: Gilbert LI, Iatrou K, Gill SS (Eds), Comprehensive Molecular Insect Science, 2005. vol.4.Elsevier, Oxford, pp: 1-77.[9]Feyereisen R, Koener JF, Farnsworth DE, Nebert DW.Isolation and sequence of cDNA encoding a cytochrome P450 from an insecticide-resistant strain of the housefly, Musca domestica[J].Proc Natl Acad Sci U S A., 1989, 86:1465-1469[10]高瑞娜, 卫正国, 张婷, 王瑞娴, 赵国栋, 李兵， 沈卫德.蜕皮激素诱导下家蚕 基因家族的表达变化[J].昆虫学报, 2010, 53(9):943-948[11]Guittard E1, Blais C, Maria A, Parvy JP, Pasricha S, Lumb C, Lafont R, Daborn PJ, Dauphin-Villemant C.CYP18A1,a key enzyme of Drosophilasteroid hormone inactivation,is essential for metamorphosis[J].Dev. Biol., 2011, 349(1):35-45[12]Hajime Ono, Katsuhisa Ozaki, Hiroshi Yoshikawa.Identification of cytochrome P450 and glutathione-S-transferase genes preferentially expressed in chemosensory organs of the swallowtail butterfly,Papilio xuthus L[J].Insect Biochemistry and Molecular Biology, 2005, 35(8):837-846[13]Hossain M1, Shimizu S, Matsuki M, Imamura M, Sakurai S, Iwami M.Expression of 20-hydroxyecdysone-induced genes in the silkworm brain and their functional analysis in post-embryonic development[J].Insect Biochemistry and Molecular Biology, 2008, 38(11):1001-1007[14]Jou?en N, Agnolet S, Lorenz S, Sch?ne SE, Ellinger R, Schneider B, Heckel D.Resistance of Australian Helicoverpa armigera to fenvalerate is due to the chimeric P450 enzyme CYP337B3[J].Proc Natl Acad Sci U S A., 2012, 109(38):15206-15211[15]Lee SH, Kang JS, Min JS, Yoon KS, Strycharz JP, Johnson R, Mittapalli O, Margam VM, Sun W, Li HM, Xie J, Wu J, Kirkness EF, Berenbaum MR, Pittendrigh BR, Clark JM.Decreased detoxification genes and genome size make the human body louse an efficient model to study xenobiotic metabolism[J].Insect Mol Biol., 2010, 19(5):599-615[16]Li X, Schuler MA, and Berenbaum MR.Jasmonate and salicylate induce expression of herbivore cytochrome P450 genes.Nature, 2002, 419(6908):712-715[17]Ma R, Cohen M B, Berenbaum M R, Schuler MA.Black swallowtail (Papilio polyxenes) alleles encode cytochrome P450s that selectively metabolize linear furanocoumarins.Archives of biochemistry and biophysics, 1994, 310(2):332-340[18]Maibeche-Coisne M, Merlin C, Francois MC, Porcheron P, Jacquin-Joly E.P450 and P450 reductase cDNAs from the moth Mamestra brassicae: cloning and expression patterns in male antennae[J].Gene, 2005, 346(14):195-203[19]Nelson DR, Zeldin DC, Hoffman SMG, Maltais LJ, Wain HM, Nebert DW.Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternative-splice variants.Pharmacogenetics, 2004, 14:1-18[20]Petersen RA, Zangerl AR, Berenbaum MR, Schuler MA.Expression of CYP6B1 and CYP6B3 cytochrome P450 monooxygenases and furanocoumarin metabolism in different tissues of Papilio polyxenes (Lepidoptera: Papilionidae).Insect biochemistry and molecular biology, 2001, 31(6-7):679-690[21]Pottier MA, Bozzolan F, Chertemps T, Jacquin-Joly E, Lalouette L, Siaussat D, Ma?bèche-Coisne M.Cytochrome P450s and cytochrome P450 reductase in the olfactory organ of the cotton leafworm Spodoptera littoralis.Insect Molecular Biology, 2012, 21(6):568-580[22]Scott JG, Kasai S.Evolutionary plasticity of monooxygenase-mediated resistance[J].Pesticide biochemistry and physiology, 2004, 78(3):171-178[23]Sztal T, Chung H, Berger S, Currie PD, Batterham P, Daborn PJ.A Cytochrome P450 Conserved in Insects Is Involved in Cuticle Formation[J].PLoS ONE, 2012, 7(5):e36544-[24]The International Silkworm Genome Consortium.The genome of a lepidopteran model insect, the silkworm, Bombyx mori.Insect Biochem. Mol. Biol., 2008, 38:1036-1045[25]Tribolium Genome Sequencing Consortium.The genome of the model beetle and pest Tribolium castaneum[J].Nature, 2008, 452:949-955[26]Wang BJ, Shahzad MF, Zhang Z, Sun H, Han P, Li F, Han Z.Genome-wide analysis reveals the expansion of Cytochrome P450 genes associated with xenobiotic metabolism in rice striped stem borer,Chilo suppressalis.Biochem Bioph Res Co., 2014, 443(2):756-760[27]Willinghama AT, Keilb T.A tissue specific cytochrome P450 required for the structure and function of Drosophila sensory organs.Mechanisms of Development, 2004, 121(10):1289-1297[28]Yamamoto K, Ichinose, H, Aso Y, Fujii H.Expression analysis of cytochrome P450s in the silkmoth,Bombyx mori.Pesticide Biochem. Physiol., 2010, 97(1):1-6[29]Zhan S, Merlin C, Boore J L, Reppert SM.The monarch butterfly genome yields insights into long-distance Migration. Cell, November, 2011, 147(5):1171-1185 |
| [1] |
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