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| Genetic Diversity and Association Analysis of SSR Markers with Leaf Stripe Resistance in Barley(Hordeum vulgare) |
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Abstract To understand the genetic diversity of barley(Hordeum vulgare) and identify SSR markers associated with resistance to barley leaf stripe, 100 pairs of SSR markers were used to detect the diversity of 86 barley entries. A total of 269 alleles were detected with an average alleles per locus of 2.69 and a range of 2~5. The allele frequency varied from 0.012 to 0.988, and the Shannon's index varied from 0.063 to 1.385. The genetic similarity ranged from 0.542 to 0.965. The polymorphism information content (PIC) value ranged from 0.023 to 0.737, with an average of 0.357. The barley entries were divided into 5 subgroups based on their population structure. Five markers were found to associate with barley leaf stripe resistance under general linear model(GLM)program, and the rate of phenotypic variation explained ranged from 6.20% to 11.15%. Two SSR markers, TACMD and MGB317, showed a significant association with resistance to barley leaf stripe (P＜0.01), which explained 9.24% and 9.45% of the phenotypic variation. Three markers were associated with resistance to barley leaf stripe under mixed linear model(MLM) programe, the rate of phenotype variation explained ranged from 4.57% to 17.63%. This study provides some theoretical basis to barley leaf stripe resistant breeding.
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Received: 15 July 2014
Published: 13 January 2015
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