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| Breeding of Transgenic Maize with Resistance to the Asian Corn Borer (Ostrinia furnacalis) and Tolerance to Glyphosate |
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Abstract 5-enolpyruvyl-shikimate-3-phosphate synthase (epsps) is a new gene of high resistance to glyphosate and Bt cry1AcM as an insect-resistent gene can expresse at high level in monocotyledon. In order to breed transgenic maize with stacking traits of insect-resistance and glyphosate-tolerance, the plant expression vector pCAMBIA1302-P35S::epsps-Tnos-Pubi::cry1AcM-Tnos was constructed and then introduced into maize (Zea mays) inbred lines by Agrobacterium mediated shoot apical meristem transformation. After herbicide screening, survived plants were confirmed by PCR, and the expression of cry1AcM gene in plants was detected by RT-PCR and Western blot, respectively. The resistance of transgenic plants to Asian corn borer(Ostrinia furnacalis (Guenée)), was examined by indoor and field experiments. And the tolerance of transgenic plants to glyphosate was evaluated by the field trials. The analysis of transgenic maize resistances demonstrated that the plants were highly resistant to herbicide and the Asian corn borer. We obtained 6 transgenic maize lines with genetic stability, highly Asiatic corn borer-resistance and glyphosate-tolerance, which achieved the criterion of actual application. Among them lines Z1~Z3 came from elite inbred line Zheng58, and lines C1~C3 came from elite inbred line Chang7-2. In summary, the introduction of cry1AcM and epsps genes into maize elite inbred lines endued the Asiatic corn borer-resistance and glyphosate-tolerance of transgenic plants, which created new corn transgenic lines with excellent complex traits. It is very useful for large-scale planting of the insect-resistance and glyphosate-tolerance transgenic maize in China.
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Received: 09 June 2014
Published: 06 January 2015
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