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| cDNA Cloning of Maize Granule-bound Starch Synthase Gene and Construction of Plant Expressing Vector |
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Abstract Two pairs primers were designed according to the already known granule-bound starch synthase (GBSS) gene sequence, one of them was used for pointing mutation. First by means of pointing mutation、RT-PCR、amalgamation PCR methods with reverse Transcription cDNA first chain as the template, the all cNDA (1818bp) fragment of maize GBSS gene was cloned from the total RNA of isolated from 15d maize Endosperm after pollination. The fragments have 99.72％ homeologous identical to the already reported sequence. Then the plant expressing vector pBI-Gt1-GBSS of containing NPT‖selective mark gene was constructed by using Endosperm-specific Promoter Gt1 as primer, NOS-ter as the teminater. It was looked forward to realizing the abundant expression of GBSS gene in maize endosperm in specific period.
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Received: 20 November 2006
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