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Abstract The expression of many induced genes is controlled by specific transcription factors and cis-acting element. Dehydration-responsive element (DRE) is very important in the regulation of the expression of drought and low-temperature responsive genes. DRE-binding transcription factors from Arabidopsis have been described. While research on DRE-binding transcription factors tree plants is scarce. Yeast one-hybrid approach is a powerful method for the searching for sequence-specific DNA binding transcription factors. Construction of DRE-containing report yeast strain is the first step for finding DRE-binding transcription factors from tree plants through yeast one-hybrid approach. This paper reports the construction of the DRE-containing report yeast strain. Our preliminary data show that the report strain we constructed is suitable for the screening for DRE-binding transcription factors from tree plants. Our work should be helpful for the improving of the stress-resistance features of tree plants.
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Received: 01 June 2006
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