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| Activity of inducible expression of Prd29A promoter in wheat and transformation of Escherichia coli trehalose-6-phosphate synthase gene |
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Abstract Using the gus gene as reporter gene, inducible expression activity in wheat calli of Prd29A promoter was measured by transient expression. The results showed that Prd29A promoter had expression activity under the salt-stress conditions in wheat. Based on the experiment above, we introduced Escherichia coli trehalose-6-phosphate synthase gene(otsA)into NC239 wheat variety through pollen tube pathway, and some otsA transgenic plants were obtained by PCR、Southern blot and trehalose amount analysis.
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Received: 08 October 2006
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