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Abstract Abstract: The avian reovirus (ARV) σ3 gene DNA fragment was labeled with Digoxigenin as cDNA probe for detection of ARV in chicken tissue and feather tips in Dot blot hybridization. As little as 1.6pg of ARV RNA could be detected with the DIG-labeled probe. Chickens were infected with ARV by inoculating at one-day old and in-contact infection. ARV could be detected in most tissues of inoculated chickens 24 hr post infection(PI) and spread to un-inoculated birds by contact rapidly. Positive rates in feather tips were correlated with other tissues. The result demonstrated that the dot blot with DIG probe to detect ARV in feather tips is a useful method for determination of ARV infection and spread in chickens, and it could be helpful when a large number of samples should be tested.
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Received: 20 November 2006
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