|
|
|
| Production of a Rabbit Monoclonal Antibody forβ-Adrenergic Agonist Ractopamine Detection |
| |
| |
|
|
|
|
Abstract A rabbit monoclonal antibody(RabMab) was generated toward theβ-Adrenergic agonist Ractopamine. Bovine serum albumin(BSA) and ovalbumin(OVA) were used as carrier proteins and Ractopamine-glutarate- BSA was used as the antigen. The WHB dark-eye rabbits were used as antibody generation animals. B lymphocytes from immuned rabbits and 240E myeloma cells were fused to generate hybridoma cells. Supernatant from clone A3-2-6 was used for the development of an immunoassay. The selected antibody was specific for ractopamine with an IC50 of 4.09ng/ml. The linear range of the inhibition rate vs log ractopamine competition curve generated in PBST was 0.1～100ng/ml. β-adrenergic agonists which had similar structures to ractopamine such as clenbuterol hydrochloride, isoxsuprine, epinephrine, norepinephrine, isoprenaline, salbutamol and dopamine showed no cross-reactivity. In conclusion, a highly specific RabMab to ractopamine hydrochloride was developed that could be used in indirect competitive ELISA screening assays.
|
|
Received: 31 March 2006
|
|
|
|
|
|
|
