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Abstract Abstract: Pig myoblasts were obtained by pronase digestion and identified by Desmin immunohistochemistry and the mRNA expression of MyoD and MyoG. The effects of leptin on the expression of thermogenesis-related genes PGC-1αand UCPs were determined by RT- PCR. It was showed that 30 nmol/L and 100nmol/L leptin promote the mRNA expression of PGC-1αin myoblasts. The effect of 100nmol/L Leptin treated for 24h was significantly higher than that of 30 nmol/L Leptin for 12h(p<0.05). Moreover, 30 nmol/L leptin markedly promoted the UCP2 mRNA expression of myoblasts, and it was higher when treated for 24h than that of 12h (p<0.05). The effect 100nmol/L leptin on UCP2 mRNA expression was significantly lower than that of 30 nmol/L Leptin in 12h and 24h, but markedly higher than that of control(p<0.05). Additionally, the mRNA expression of UCP3 was not significantly different when treated by 30 nmol/L and 100nmol/L leptin compared to control in myoblasts(p<0.05). It suggests that leptin stimulate energy consumption by promoting the mRNA expression of PGC-1αand UCP2 in myoblasts.
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Received: 16 November 2006
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