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| Construction of Recombinant Strain Expressing mSTⅠ-linker-LTB Fused Protein of Enterotoxigenic Esherichia coli and Its Immuonoprotection Test |
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Abstract The fused gene of Enterotoxigenic Esherichia coli mSTⅠ-linker-LTB was construction and was cloned into plasmid pET32a(+).The recombinatant plamsid was transformed into the expression host strain E.coli BL21（DE3）. After 5 hour induced by 1mM IPTG at 30℃, the fusion protein which molecular weight was about 37K was at the highest level. Its content was about 30% of that of total bacterial proteins. The fusion protein was mainly in inbody and was positive by Western blot. It was no the biological toxicity of the native STI demonstrated by the Suckling Mouse Assay. The purified recombinant protein emulsificated with mineral oil offered ICR mouse 83.3%、100%、66.7% and 66.7% protection against the challenge of Enterotoxigenic Esherichia coli K88、K99、987P and F41,respectively.
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Received: 21 September 2006
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