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Abstract SRAP is a new molecular marker which aimed for the amplification of open reading frames(ORFs). It is simplicity, reliability, moderate throughput ratio and has not the specie-specific character. It had been widely used for gene tagging, germplasm collection,genetic diversity,map construction and comparing genome analysis. Factors influencing SRAP-PCR analysis were studied using three pear cultivars. A reliable,effective and reproductive PCR reaction system for detecting SRAP was developed. Each 20μL PCR reaction mixture consisted of template DNA 30ng, 2.0mmol/L of MgCl2, 200μmol/L of dNTPs, 30ng of primer and 1 unit of Taq polymerase.
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Received: 20 November 2006
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