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Abstract It is important to understand the mechanism of wheat tolerance to drought, to investigate the wheat transcriptional profiling drought stress, a drought tolerance variety-Hanxuan 10 was treated by PEG6000,RNA samples were collected at 0, 1, 6, and 24 hours respectively, influence dye-labeled complimentary DNA were used to hybridize with the whole genome rice gene chip, which contains over 60K oligos designed based on rice sequence. Data analysis indicated that the number of differentially expressed genes was increased as longer PEG treatment, functional category analysis results indicate that number of energy metabolism pathway-related gene was increased and account for more portion of differentially expressed genes. Most of the photosynthesis related genes was up-regulated but interestingly, Psbr and Rubisco coding genes were down-regulated, suggesting their potential role in drought tolerance response.
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Received: 30 January 2007
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