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| Cloning and Characterization of A full-length cDNA of WRKY transcription factor gene in Brassica campestris ssp.chinensis |
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Abstract PCR primers were designed based on the conserved domain of Arabidopsis WRKY transcription factor, the full-length cDNA of WRKY transcription factor was cloned from Brasssica campestris ssp.chinensis using RT-PCR and RACE techniques. Sequence analysis indicated that BcWRKY1 gene(GenBank accession number: AY836002) consisted of 980 nucleotides(nt), and deduced amino acid sequence containing 285 amino acids. Further comparison showed its identity was 74% and 59% to AtWRKY18 gene and AtWRKY60 gene of Arabidopsis, respectively. The deduced amino acid sequence of WRKY1 gene has lower homology with other plant WRKY genes. Southern-blot analysis indicated there was a single copy in genome of B. campestris ssp.chinensis. Semi-quantitative RT-PCR demonstrated that the corresponding mRNA of WRKY1 was accumulated most abundantly 2h-8h after treatment upon 2mmol/L SA, and 6h-12h after infection by Peronospora parasitica, respectively.
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Received: 30 January 2007
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