bFGF Gene Expression in Goat Ovary Follicular Cells and Partial Sequence Analyses of bFGF cDNA
Wang Baoli1,2 Zhang Yong1
(1. Institute of Biotechnology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China; 2. College of Life Sciences, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China)
Abstract Abstract: A RT-PCR method for detecting gene expression of the goat basic fibroblast growth factor (bFGF) was set up. The expressions of bFGF gene in goat ovarian follicle, cumulus-oocyte complexes (Caprane) (COCs), granulosa cells(GCs) and theca follicular cells were observed, respectively. The results indicated that all these cells expressed bFGF mRNA. Meanwhile, the bFGF cDNA was amplified. The isolated fragment (283 nt) was cloned and sequenced. Comparison of the nucleotide sequence indicated 99.3% similarity to the bovine bFGF gene and 95.4% similarity to the human bFGF gene.
