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| Construction and Expression of a Brassica napus Myrosinase in Escherichia coli |
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Abstract The myrosinase gene was amplified from total RNA by RT-PCR and inserted into Escherichia coli expression vector pGEX-4T-1.The result of sequencing was EF583560 in GenBank.The amino acid sequence showed that the homology was 99% comparing with a length of published amino acid sequence of Q00326.The recombinant protein was highly expressed at 91KDa by IPTG induced in Escherichia coli.
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Received: 11 October 2007
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