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| Cloning of 3′-end full length of cDNA encoding for MT-I/Ⅲ and analyzing of the sequence in Yak |
| Bin LIU Jian-ping WU Bin-yun MA Feng-qin GAO |
| 内蒙古呼和浩特市鄂尔多斯西街内蒙古农牧科学院畜牧所 |
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Abstract Abstract: In the present study, the domestic yak (B.Grunniens) metallothionein-I and Ⅲ cDNA 3′-end full length sequences (331bp, 378bp) were amplified and cloned by RT-PCR and 3′-RACE using the primer YMT-ⅠSP, YMT-ⅢSP and M13 Primers M4, in which included MT-I and MT-Ⅲ cDNA coding sequences (183bp, 207bp) and AATAAA and Poly（A）in the end. The cDNA sequences of MT-I and MT-Ⅲ were subjected to Blastn searching in CBI and the results showed that the nucleotide sequences of yak MT-I and MT-Ⅲ between different species mammal are comparatively conservative. The yak cDNA sequence coding for yak MT-I protein is composed of 61 amino acids, including 20 Cysteine. There are lots of conserved tripetide, such as C-X-C, C-C-X-C-C, C-X-X-C, etc.. The yak cDNA sequence coding for yak MT-Ⅲ protein is composed of 68 amino acids, including 19 Cysteine, which has the same conserved tripetide with MT-Ⅰ, and MT-Ⅲ has also its own particular conserved tripetide, such as T, CPCP,GEGAEA, etc.. They are all comparatively conservative in their evolution.
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Received: 31 July 2007
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Corresponding Authors:
Jian-ping WU
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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