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Abstract Abstract：Monoclonal antibodies against Pasteurella multocida toxin(PMT) were produced by using E.coli expressed recombinant PMT proteins as immunogens.on the basis of recombinant protein expressed by E.coli and HRP-labled monoclonal antibody against Pasteurella multocida toxin,a competitive ELISA was developed to detect antibodies against.Pasteurella multocida toxin.Optimium conditions of the ELISA Were developed as following：the concentration of recombinant for coating ELISA plates is 223 ng /mL;the best dilution of serum to be tested was 1:2; the working titer for HRP-labelled monoclonal antibody was 1:3200;>50% of inhibition rate was the positive judging standard.A total of 82 serum samples were detected in parallel by both the competitive ELISA and in vitro neutralization assay.40.2％ of them were detected as positive by competitive ELISA,while 36.6％ of them were positive by in vitro neutralization assay.The coincidence rate of the two assays is 91.5%.These results showed that the cELISA has the advantage of higher sensitivity，specificity，reproducibility and stability.It would be very useful in diagnosis，surveillance of PMT antibodies and epidemiological survey.
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Received: 06 December 2007
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