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| Construction of Fusion Gene RSV CP and Rubisco SSU Leader Peptide of Rice Chloroplast and Its Prokaryotic Expression |
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Abstract The coat protein (CP) gene of Rice stripe virus (RSV) and Rubisco SSU leader peptide gene of rice chloroplast were amplified by PCR。Depending on the expression vector pGEX-4T-1 and pET-29a, fusion gene PR-CP and PR-S-CP was constructed. The two fusion genes were confirmed correct by sequencing and analysis. The recombinant pGEX-PR-CP and pET-PR-S-CP were transformed into E. coli BL21 (DE3), and then induced to express by IPTG. The expression products of fusion gene were identified by SDS-PAGE electrophoresis and Western bolt. The result suggested that the fusion proteins were the same as we expected and could be specifically recognized by the antibody to RSV CP. This suggested that RSV CP gene in the fusion gene expressed correctly and maintained its antigen activity, so we could conclude that the expression products of fusion gene in the organism maybe could maintain its structure and function separately.
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Received: 18 September 2007
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