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| The Gene Encoding CuZn-Superoxide Dismutase from Bacillus cereus 905 and its expression in Escherichia coli |
| Xiao-Dan Mo Qi Wang |
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Abstract Bacillus cereus strain 905 isolated from wheat is an endophytic beneficial bacterium that can promote plant growth. In this study, the gene encoding CuZn-SOD in B. cereus 905 was cloned by using of PCR to analyze the toxicity of active oxygen species (AOS) to the bacteria during the colonization in plant. The sequence analysis shows that this gene contains 537 base pairs which encoding 179 amino acid residues. For studying the encoded peptide characteristics, the expression vector pET-sodC in Escherichia coli BL21(DE3) was constructed. Results showed the induced protein with IPTG was confirmed as a superoxide dismutase by dyed with NBT. Finally, the gene in B. cereus 905 encoding CuZn-SOD was identified on the basis of inhibited SOD activity by potassium cyanide (KCN) and hydrogen peroxide (H2O2).
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Received: 11 October 2007
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Corresponding Authors:
Qi Wang
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