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| Study about A New Strain WZ-9 of Bacillus thuringiensis with Insecticidal Activity Against Henosepilachna vigintioctomaculata |
| Ping SONG |
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Abstract A high toxicity Bacillus thuringiensis (Bt) strain WZ-9 against larvae of Henosepilachna vigintioctomaculata was isolated from soil of Hebei province. Bipyramidal inclusions could be observed by scanning electron microscopy. SDS-PAGE analysis indicated that the strain produced a 130 kDa protein. Growth cycle of this strain was 24h, and pH of the culture varied with the strain growth. The bioassay results showed that the WZ-9 strain was highly toxic to the 2 instar larvae of H. vigintioctomaculata.. Correct mortality is 100% at 72h, and LC50 is 2.95×107cell/mL. Genotype identify showed this strain had cry7 gene. Sequence analysis showed that the encoding gene contained a ORF of 3414 bps and encoded 1138 amino acids residues. The deduced amino acid sequence is 99.65% identical to that of the reported Cry7Ab2 sequences. By Bt δ-endotoxin nomenclature committee this gene was designated as Cry7Ab3 with accession number BI 1015188 in GenBank database.
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Received: 11 October 2007
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Corresponding Authors:
Ping SONG
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