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| Effects of Methionine and Methionylmethionine on Expression of Casein αs1 in Cultured Bovine Mammary Epithelial cells |
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Abstract This study was carried out to investigated the effects of methionine and methionylmethionine on cultured bovine mammary epithelial cells. The methionine-modified medium consisted of 0-100 μg/ml of methionine. The expression of casein αs1 gene was increased with adding level of methionine from 0 to 60 μg/ml, and then decreased. The estimated optimal adding level of methionine was at 57 μg/ml. Expression of casein αs1 gene was much higher when 60 μg/ml methionylmethionine was added instead of methionine (P<0.01). These results indicated that mammary epithelial cells can utilize methionylmethionine for milk protein synthesis with a higher efficiency than methionine.
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Received: 03 April 2006
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