|
|
|
Construction of Recombinant DNA Plasmid Co-expressing Capsid Protein of
Porcine circovirus Type 2 and Porcine Granulocyte-macrophage Colony-stimulating Factor and Its Expression In vitro |
| SONG Qin-ye;YANG Han-chun;GUO Xin;CHEN Yan-hong;CHA Zhen-lin |
| Key Laboratory for Preventive Veterinary Medicine, Ministry of Agriculture, China Agricultural University, Beijing 100094, China |
|
|
|
|
Abstract The orf2 gene of Porcine circovirus type 2 (PCV2) and porcine granulocyte-macrophage colony-stimulating factor (gm-csf) were inserted into pIRESneo to construct recombinant plasmid pIRES-orf2/gm-csf. The recombinant plasmid pIRES-orf2/gm-csf was then transfected transiently into COS7 cells, the expressions of orf2 and gm-csf genes were confirmed by indrect immunofluorscence assay (IFA) and colony-stimulation forming assay. The results showed that the transfected cells could display specific immunofluorscence and the supernatant of cell culture had the activity for stimulating the formation of porcine bone marrow cell colonies. The successful construction of pIRES-orf2/gm-csf co-expressing PCV2 orf2 and gm-csf genes provides foundation for the further study of the PCV2 DNA vaccine.
|
|
Received: 22 April 2005
|
|
Corresponding Authors:
YANG Han-chun
|
|
|
|
|
|
|
