Screening and Verification of Minor Genes Related to Fatty Acid Metabolism in High Oleic Acid Rapeseed (Brassica napus)
WANG Xiao-Dan, XIAO Gang, ZHANG Zhen-Qian*, GUAN Chun-Yun
Southen Regional Collaborative Innovation Center for Grain and Oil Crops/Hunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Hunan Agricultural University, Changsha 410128, China
Abstract：The genetic mechanism of oleic acid in rapeseed (Brassica napus) is complex. The main gene FAD2 (fatty acid desaturase 2) is studied mostly, but little about minor genes now. In order to discover new related genes and promote the research of fatty acid metabolism in high oleic acid rapeseed, 20 high oleic acid rapeseed strains were used as materials in this study. Not only were their fatty acid composition tested by gas chromatography (GC), but also the expressions of OPR (1,2-oxo-phytodienoic acid reductase) and AT(acetyltransferase) gene, which screened from microRNA sequencing and related to fatty acid metabolism, were determined by qRT-PCR. Meanwhile, the relationship between the expression of the 2 genes and the fatty acid composition were analyzed. The results showed that the content of oleic acid was negatively correlated with linoleic acid and α-linolenic acid. Linoleic acid, α-linolenic acid and palmitic acid were positively correlated with each other. The expression patterns of OPR and AT genes were similar during the whole growth period. Their expression increased gradually at seedling stage, the highest expression was in blooming flowers at flowering stage, and decreased gradually at pod stage, while the expression level of OPR gene was much lower than that of internal reference gene, and it was opposite with AT. The analysis between expression of 2 genes and fatty acid composition was found that AT gene was significantly or extremely significantly correlated with oleic acid, linoleic acid and α-linolenic acid in leaves of 5~6 leaf stage, budding stage and selfed seeds of 35 d; OPR was positively correlated with oleic acid and negatively correlated with linoleic acid and linolenic acid during the whole pod stage. The 2 genes might be related with oleic acid synthesis as the minor genes. Besides, AT could be used for early screening of high oleic acid materials. The results of this study could promote the research of molecular mechanism in high oleic acid rapeseed.
王晓丹, 肖钢, 张振乾, 官春云. 高油酸油菜脂肪酸代谢相关微效基因筛选及验证[J]. 农业生物技术学报, 2019, 27(7): 1171-1178.
WANG Xiao-Dan, XIAO Gang, ZHANG Zhen-Qian, GUAN Chun-Yun. Screening and Verification of Minor Genes Related to Fatty Acid Metabolism in High Oleic Acid Rapeseed (Brassica napus). 农业生物技术学报, 2019, 27(7): 1171-1178.
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