|
|
|
| Bioinformatics and Expression Analysis of StERF5 Gene in Potato (Solanum tuberosum) |
| WANG Rui1, PU Xue1, WANG Kai-Tong1, ZHANG Huan-Huan1,2, ZHANG Ning1,2,*, SI Huai-Jun1,2 |
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2 State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China |
|
|
|
|
Abstract APETALA2/ethylene responsive factor (AP2/ERF) family is one of the largest transcription factor families in plants. ERF subfamily is the main member of AP2/ERF family and plays an important role in plant response to biotic and abiotic stresses. In this experiment, the bioinformatics analysis, subcellular localization and gene expression analysis of potato (Solanum tuberosum) StERF5 gene were carried out to further clarify the function of the gene. The results showed that the coding region of the gene was encoding 243 amino acids and contained an AP2 domain. The subcellular localization showed that the protein was localized in the nucleus and cell membrane. Tissue specific expression analysis showed that StERF5 gene was expressed in roots, stems and leaves, with the highest expression level in roots. There were significant differences in the expression levels of StERF5 gene in stems of different potato varieties (P<0.01). Expression of StERF5 gene was up-regulated under drought and salt treatments. This study lays the foundation for the subsequent in-depth understanding of function of StERF5.
|
|
Received: 28 January 2022
|
|
|
|
Corresponding Authors:
*ningzh@gsau.edu.cn
|
|
|
|
[1] 李荣, 冯月娟, 王舰, 等. 2021. 马铃薯StSnRK2.4基因的序列分析及其在非生物胁迫下的表达分析[J].分子植物育种 19(22): 7327-7336.
(Li R, Feng Y J, Wang J, et al.2021. Sequence and expression analysis under abiotic stress of potato StSnRK2.4 gene[J]. Molecular Plant Breeding, 19(22): 7327-7336.)
[2] 梁丽娜. 2017. 干旱胁迫下马铃薯酵母cDNA文库构建及筛选[D].硕士学位论文,甘肃农业大学, 导师: 张宁, pp. 5.
(Liang L N. 2017. Yeast cDNA library construction and ERF transcription factor screening of potato (Solanum tuberosum L.) under droughr stress[D]. Thesis for M.S., Gansu Agricultural University, Supervisor: Zhang N, pp. 5.)
[3] 邵欣欣, 李涛, 李植良, 等. 2015. 茄子AP2/ERF转录因子的鉴定及胁迫条件下的表达分析[J].植物生理学报, 51(11):1901-1918.
(Shao X X, Li T, LI Z L, et al.2015. Genome-wide identification and expression analysis in oxidative stress of AP2/ERF gene family in eggplant (Solanum melongena)[J]. Plant Physiology Journal, 51(11): 1901-1918.)
[4] 王芳芳. 2021. 马铃薯StERF109基因功能的鉴定[D].硕士学位论文,甘肃农业大学,导师: 张宁, pp. 63.
(Wang F F. 2021. Identification of StERF109 gene function in potato[D]. Thesis for M.S., Gansu Agricultural University, Supervisor: Zhang N, pp. 63.)
[5] 张麒, 陈静, 李俐, 等2018. 植物AP2/ERF转录因子家族的研究进展[J]. 生物技术通报, 34(8): 1-7.
(Zhang L, Chen J, Li L, et al.2018. Research progress on plant AP2/ERF transcription factor family[J]. Biotechnology Bulletin, 34(8): 1-7.)
[6] An J P, Zhang X W, Bi S Q, et al.2020. The ERF transcription factor MdERF38 promotes drought stress-induced anthocyanin biosynthesis in apple[J]. The Plant Journal, 101(3): 573-589.
[7] Chuang H W, Harnrak A, Chen Y C, et al.2010. A harpin-induced ethylene-responsive factor regulates plant growth and responses to biotic and abiotic stresses[J]. Biochemical and Biophysical Research Communications, 402(2): 414-420.
[8] Charfeddine M, Charfeddine S, Ghazala I, et al.2019. Investigation of the response to salinity of transgenic potato plants overexpressing the transcription factor StERF94 [J]. Journal of Biosciences, 44(6): 141.
[9] Debbarma J, Sarki Y N, Saikia B, et al.2019. Ethylene response factor (ERF) family proteins in abiotic stresses and CRISPR-Cas9 genome editing of ERFs for multiple abiotic stress tolerance in crop plants: A review[J]. Molecular Biotechnology, 61(2): 153-172.
[10] Dilnur T, Manabayeva S A.2021. Employing CRISPR/Cas technology for the improvement of potato and other tuber crops[J]. Frontiers in Plant Science, 12: 747476.
[11] Dong L D,Cheng Y X,Wu J J, et al.2015. Overexpression of GmERF5, a new member of the soybean EAR motif-containing ERF transcription factor, enhances resistance to Phytophthora sojae in soybean[J]. Journal of Experimental Botany, 66(9): 2635-2647.
[12] Feng K, Hou X L, Xing G M, et al.2020. Advances in AP2/ERF super-family transcription factors in plant[J]. Critical Reviews in Biotechnology, 40(6): 750-776.
[13] Han D G, Han J X, Xu T L, et al.2021. Overexpression of mberf12, an ERF gene from Malus baccata (L.) Borkh, increases cold and salt tolerance in Arabidopsis thaliana associated with ROS scavenging through ethylene signal transduction[J]. In Vitro Cellular & Developmental Biology-Plant, 57(5): 1-11.
[14] Jin Y, Pan W Y, Zheng X F, et al.2018. OsERF101, an ERF family transcription factor, regulates drought stress response in reproductive tissues[J]. Plant Molecular Biology, 98(1-2): 51-65.
[15] Lee D K, Jung H, Jang G, et al.2016. Overexpression of the OsERF71 transcription factor alters rice root structureand drought resistance[J]. Plant Physiology, 172(1): 575-588.
[16] Licausi F, Ohme-Takagi M, Perata P.2013. APETALA2/ethylene responsive factor (AP2/ERF) transcription factors: Mediators of stress responses and developmental programs[J]. New Phytologist, 199(3): 639-649.
[17] Liu C X, Zhang T Z.2017. Expansion and stress responses of the AP2/EREBP superfamily in cotton[J]. BMC Genomics, 18(1), 118.
[18] Liu W, Wang Y, Gao C.2014. The ethylene response factor (ERF) genes from Tamarix hispida respond to salt, drought and aba treatment[J]. Trees, 28(2): 317-327.
[19] Ma L F, Hu L X, Fan J B, et al.2017. Cotton GhERF38 gene is involved in plant response to salt/drought and ABA[J]. Ecotoxicology, 26(6): 841-854.
[20] Mizoi J, Shinozaki K, Shinozaki K Y, et al.2012. AP2/ERF family transcription factors in plant abiotic stress responses[J]. Biochimica et Biophysica Acta, 1819(2): 86-96.
[21] Park H Y, Seok H Y, Woo D H, et al.2011. AtERF71/HRE2 transcription factor mediates osmotic stress response as well as hypoxia response in Arabidopsis[J]. Biochemical and Biophysical Research Communications, 414(1): 135-141.
[22] Riechmann J L, Heard J, Martin G, et al.2000. Arabidopsis transcription factors: Genome-wide comparative analysis among eukaryotes[J]. Science, 290(5499): 2105-2110.
[23] Sakuma Y, Liu Q, Dubouzet J G, et al.2002. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration-and cold-inducible gene expression[J]. Biochemical and Biophysical Research Communications, 290(3): 998-1009.
[24] Sun W J, Ma Z T, Chen H, et al.2019. MYB gene family in potato (Solanum tuberosum L.): Genome-wide identification of hormone-responsive reveals their potential functions in growth and development[J]. International Journal of Molecular Sciences, 20(19): 4847.
[25] Xu Z S, Xia L Q, Chen M, et al.2007. Isolation and molecular characterization of the Triticum aestivum L. ethylene-responsive factor 1 (TaERF1) that increases multiple stress tolerance[J]. Plant Molecular Biology, 65(6): 719-732.
[26] Yang H, Yu C, Yan J, et al.2014. Overexpression of the Jatropha curcas JcERF1 gene coding an AP2/ERF-type transcription factor increases tolerance to salt in transgenic tobacco[J]. Biochemistry, 79(11): 1226-1236.
[27] Zhang G, Chen M, Chen X P, et al.2010. Isolation and characterization of a novel EAR-motif-containing gene GmERF4 from soybean (Glycine max L.)[J]. Molecular Biology Reports, 37(2): 809-818.
[28] Zhang G Y, C Ming, Li L C, et al.2009. Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco[J]. Journal of Experimental Botany, 60(13): 3781-3796.
[29] Zhao Y L, Chang X, Qi D Y, et al.2017. A novel soybean erf transcription factor, gmerf113, increases resistance to Phytophthora sojae infection in soybean[J]. Frontiers in Plant Science, 8(47): 299. |
|
|
|
