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| Correlation Study on Polymorphism of Ovoinhibitor (OIH) and Gonadotropin-inhibitor Hormone (GnIH) with Laying Performance in Jinding Duck (Anas platyrhynchos domestica) |
| CAO Yong-Qing1,2, ZENG Tao2, LIU Guo-Fa3, ZHOU Wei3, SHI Fang-Xiong1*, LU Li-Zhi2* |
1 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2 Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China;
3 Zhoukou Guiliu Duck Breeding Co, Ltd., Zhoukou 461300, China |
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Abstract Jinding duck (Anas platyrhynchos domestica) is a good breed of laying duck in China. It has many advantages, such as more eggs, strong resistance to stress and so on. It is excellent materials for breeding of new breed (matching line) of laying duck. In this study, 487 Jinding ducks were selected as the research objects, and the ovoinhibitor gene (OIH) and gonadotropin inhibitor gene (GnIH) were detected by DNA direct sequencing for SNP and genetic analysis, and the correlation with the age at first egg, egg numbers at 300 d and 500 d old was analyzed. The results showed that there were 7 polymorphic loci in the OIH gene exon and 4 polymorphic loci in the GnIH gene exon of Jinding duck, and all the loci produced 3 genotypes, 7 of which were missense mutations. The results of genetic analysis showed that all the 11 loci were in Hardy-Weinberg equilibrium (P>0.05), 8 of them were moderate polymorphism (0.50>PIC>0.25), 3 of them were low polymorphism (PIC<0.25). The results of linkage disequilibrium analysis showed that G2344A and T2363C, C2385T and C5749T were complete linkage disequilibrium, G1946A, T1949C and T2218C were complete linkage disequilibrium between any two of them. The results of correlation analysis showed that the C469T locus of OIH gene was significantly correlated with the age at first egg and egg numbers at 300 d old (P<0.05), and AA was the dominant genotype; A517G, C2385T, C5749T and G5801A loci of OIH gene had significant influence on the age at first egg (P<0.05); C2089T locus of GnIH gene was significantly correlated with the egg numbers at 500 d old (P<0.05). In this study, SNPs with genetic effect on laying performance of Jinding duck were screened, which could provide reference for the establishment of candidate genes of laying performance and molecular marker assisted selection.
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Received: 15 August 2019
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Corresponding Authors:
*fxshi@njau.cn; lulizhibox@163.com
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