Bioinformatics Analysis, Subcellular Localization and Expression Analysis of OsERF103 Gene in Rice (Oryza sativa)
ZOU Jie*, LI Sheng-Qiang, LIU Xian-Jun, CHEN Gang
College of Life Science and Environmental Resources/Jiangxi Provincial Key Laboratory of Crop Growth and Development Regulation, Yichun University, Yichun 336000, China
Abstract：Ethylene responsive factors (ERFs) are plant-specific transcription factors that play important roles in plant developmental process and in response to abiotic stress. In order to explore the function of OsERF103 (GenBank No. XM_015768788) in rice (Oryza sativa) , bioinformatics methods were used to analyze the sequence characteristic of OsERF103. For subcellular localization analysis, the fusion expression vector of pBWA(V)HS-OsERF103-Glosgfp was constructed and transformed into rice protoplast, then laser scanning confocal microscopy was used to observe the subcellular localization of OsERF103 fusion protein in rice protoplast; Expression analysis of OsERF103 in different tissues and under different abiotic stress conditions was conducted by qRT-PCR. The results showed that OsERF103 was a hydrophilic and unstable protein without signal peptide and transmembrane structure, and contained an AP2 (APETALA2) domain. Phylogenetic analysis revealed the OsERF103 was evolutionarily closest to a abscisic acid repressor 1-like (ABR1-like) protein of Oryza brachyantha. The results of subcellular localization showed that OsERF103 was located in the nucleus. OsERF103 transcripts were detected in all sampled tissues at booting stage, with the highest level detected in root and weak level detected in leaf and young spike. OsERF103 transcripts were induced by high temperature, low temperature, PEG6000, high salt and abscisic acid (ABA), and differential expression patterns of OsERF103 were showed under the different treatment conditions. This study provides basic data for further exploring the biological function of OsERF103.
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